EMBO Practical Course on Analysis of High-Throughput Sequencing Data
Is it right for me?
This course is aimed at advanced PhD students and post-doctoral researchers who are applying or planning to apply high throughput sequencing technologies and bioinformatics methods in their research.
The aim of this course is to familiarize the participants with advanced data analysis methodologies and provide hands-on training on the latest analytical approaches.
What will I learn?
Lectures will give insight into how biological knowledge can be generated from RNA-seq and ChIP-seq experiments and illustrate different ways of analyzing such data. Practicals will consist of computer exercises that will enable the participants to apply statistical methods to the analysis of RNA-seq and ChIP-seq data under the guidance of the lecturers and teaching assistants.
Familiarity with the technology and biological use cases of high throughput sequencing is required, as is some experience with R/Bioconductor.
What will it cover?
The course covers data analysis of RNA-Seq and ChIP-Seq experiments.
Topics will include: short read alignment using Bowtie/BWA; data handling and visualisation, region identification, differential expression, data quality assessment and statistical analysis, using R/Bioconductor.
| Time | Topic |
|---|---|
| Sun 23 Oct 2011 - Arrive @ Wellcome Trust | |
| Day 1 - Mon 24 Oct 2011 - Short read alignment - Marco Chierici, Marco Roncador | |
| 9:00 - 9:30 | Registration and welcoming |
| 9:30 - 10:30 | Lecture: Next generation sequencing: an overview |
| 10:30 - 11:00 | Coffee break |
| 11:00 - 12:30 | Lecture: Computational tools, alignment strategies and bioinformatics challenges using Bowtie, BWA, SSAHA2, Cufflinks, SAMTools and BEDTools |
| 12:30 - 13:30 | Lunch |
| 13:30 - 17:30 | Practical: Short read alignment, analysis and visualization |
| Day 2 - Tue 25 Oct 2011 - Short read analysis with R & Bioconductor - Nicolas Delhomme | |
| 9:00 - 9:30 | Lecture: Introduction to R & Bioconductor |
| 9:30 - 10:30 | Lecture: Bioconductor packages for Short Read Analysis |
| 10:30 - 11:00 | Coffee break |
| 11:00 - 12:30 | Lecture/Practical: Bioconductor packages for Short Read Analysis (cont.) |
| 12:30 - 13:30 | Lunch |
| 13:30 - 14:30 | Lecture/Practical: Bioconductor packages for Short Read Analysis (cont.) |
| 14:30 - 16:30 | Lecture/Practical: Bioconductor packages for quality assessment |
| 16:30 - 17:30 | Lecture: SNP calling / SNP injecting |
| Day 3 - Wed 26 Oct 2011 - ChIP-seq data analysis - Kathi Zarnack, Borbala Gerle | |
| 9:00 - 10:30 | Lecture: Introduction to ChIP-Seq data and analysis (KZ, BG) |
| 10:30 - 11:00 | Coffee break |
| 11:00 - 12:30 | Lecture: ChIP-seq data analysis with Bioconductor (KZ, BG) |
| 12:30 - 13:30 | Lunch |
| 13:30 - 16:30 | Practical: ChIP-seq data analysis (KZ, BG) |
| 16:30 - 18:00 | Poster session I |
| Day 4 - Thu 27 Oct 2011 - RNA-seq data analysis I - Benilton Carvalho | |
| 9:00 - 10:30 | Lecture: Statistical concepts and methodologies for data analyses |
| 10:30 - 11:00 | Coffee break |
| 11:00 - 12:30 | Lecture: Statistical concepts and methodologies for data analyses (cont.) |
| 12:30 - 13:30 | Lunch |
| 13:30 - 17:30 | Practical: tbc |
| 19:00 | Dinner at Jesus College, Cambridge |
| Day 5 - Fri 28 Oct 2011 - RNA-seq data analysis II – John Marioni, Wolfgang Huber | |
| 9:00 - 10:00 | Lecture: Differential expression - negative binomial based models for testing and regression (WH) |
| 10:00 - 11:00 | Lecture: Allele specific expression and eQTL (JM) |
| 11:00 - 11:30 | Coffee break |
| 11:30 - 12:30 | Lecture: Alternative splicing and differential exon usage (WH) |
| 12:30 - 13:30 | Lunch |
| 13:30 - 16:30 | Practical: Differential expression (WH) |
| 16:30 - 18:00 | Poster session II |
| Day 6 - Sat 29 Oct 2011 - Data integration, advanced analysis – John Marioni, Wolfgang Huber | |
| 9:00 - 10:00 | Lecture: Clustering and classification (JM) |
| 10:00 - 11:00 | Lecture: Exploring and visualising high-dimensional data (WH) |
| 11:00 - 11:30 | Coffe Break |
| 11:30 - 12:30 | Q/A and wrap-up session |
Course materials can be downloaded from the following links:
/training/sites/ebi.ac.uk.training/files/materials/2011/111023_microarray/day1.zip
/training/sites/ebi.ac.uk.training/files/materials/2011/111023_microarray/day2.zip
/training/sites/ebi.ac.uk.training/files/materials/2011/111023_microarray/day3.zip
/training/sites/ebi.ac.uk.training/files/materials/2011/111023_microarray/day4.zip
/training/sites/ebi.ac.uk.training/files/materials/2011/111023_microarray/day5.zip
Trainers
Marco Chierici, FBK – MPBA, Trento, Italy
Marco Roncador, FBK – MPBA, Trento, Italy
Nicolas Delhomme, EMBL, Heidelberg
Kathi Zarnack, EBI
Borbala Gerle, EBI
Benilton Carvalho, CRI, Cambridge
Wolfgang Huber, EMBL, Heidelberg
John Marioni, EBI
