The Bioinformatics Roadshow - Sydney Australia
What will I learn?
This course is designed to familiarise participants with next Generation Sequence data analysis and to provide hands-on computational experience in analysis of NGS data using common analytical approaches for ChIP-seq, RNA-Seq data and de novo genome assembly.
The course covers data analysis of RNA-Seq and ChIP-Seq experiments and de novo genome assembly of small genomes. Topics will include: data quality, alignment, data handling and visualisation, region identification, transcriptome analysis and basis statistical analysis.
The practical 2 day hands-on course is designed for bench biologists, PhD students or early career postdoctoral researchers with no or basic bioinformatics experience who are planning or currently using NGS approaches in their research area.
|Day 1 - Thursday 12th July, 2012 - Introduction to NGS and ChIP-seq data analysis|
|09:00 - 09:40||Welcome and introductions to the course (Catherine Shang)
|09:40 - 10:30||Next generation sequencing overview(Sean McWilliam)|
|10:30 - 10:50||Tea/coffee break|
|10:50 - 12:25||Practical: NGS quality control and sequence alignment (Soniya Tyagi/Xi Li)|
|12:25 - 13:10||Lunch|
|13:10 - 14:00||Introduction to ChIP-seq (Myrto Kostadima/Remco Loos)|
|14:00 - 15:30||Practical: ChIP-seq analysis - peak calling and annotation (Myrto Kostadima/Remco Loos)|
|15:30 - 15:50||Coffee/Tea Break|
|15:50 - 16:40||Practical: ChIP-seq analysis - motif analysis (Myrto Kostadima/Remco Loos)|
|16:40 - 17:30||Wrap up of day 1 and discussions|
|Day 2 - Friday 13th July, 2012 - Introduction to RNA-seq and de novo genome assembly|
|09:00 - 09:10||Welcome and introductions to Day 2 (Myrto Kostadima/Remco Loos)|
|09:10 - 09:40||Introduction to RNA-seq (Myrto Kostadima/Remco Loos)|
|09:40 - 10:15||Practical: alignment and slice junction identification (Myrto Kostadima/Remco Loos)|
|10:15 - 10:40||Practical: transcriptome assembly (Myrto Kostadima/Remco Loos)|
|10:40 - 11:05||Coffee/Tea Break|
|10:05 - 12:30||Practical: differential expression analysis (Myrto Kostadima/Remco Loos)|
|12:30 - 13:15||Lunch|
|13:15 - 13:45||Introduction to de novo assembly (Matthais Haimel)|
|13:45 - 15:30||Practical: de novo assembly using velvet (part 1 and 2) (Matthais Haimel)|
|15:30 - 15:45||Coffee/Tea Break|
|15:45 - 16:45||Practical: de novo assembly using velvet (part 3) (Matthais Haimel)|
|16:45 - 17:05||Review and discussion of Velvet de novo assembly exercises (Matthais Haimel)|
|17:05 - 17:30||General course discussion and feedback survey completion (Catherine shang)
Due to the practical component of this course places are limited.
Please download the application form
Send completed application forms to firstname.lastname@example.org
The closing date for application is 27 June (12:00 noon AEST).
Successful applicants will be notified by email by 29th June this will include instructions for course payment.
Registration will be via a selection process therefore applicants should be aware that, in order to be considered for place on this course, they MUST complete the application page. Items marked * are mandatory. Incomplete registrations will not be processed.
The course cost is $150 and includes morning and afternoon tea and lunches