Introduction to Next Generation Sequencing
This course will provide an introduction to the technology, data analysis, tools and resources for next generation sequencing (NGS) data. The content is intended to provide a broad overview of the subject areas, and to highlight key resources, approaches and methodologies. Topics will be delivered using a mixture of lectures, practical sessions and open discussions. Practical work during the course will use small, example datasets and there will not be scope to analyse personal data.
This course is aimed at wet-lab biologists who are embarking upon research projects that will involve NGS and the analysis of the data generated.
Participants will require a basic knowledge of the Unix command line, Bio-Linux and the R statistical package. We recommend these free tutorials:
- Basic introduction to the Unix environment:
- Introduction and exercises for Bio-Linux 7:
- Basic R concept tutorials:
Please note: participants without basic knowledge of these resources will have difficulty in completing the practical sessions.
Syllabus, Tools and Resources
During this course you will learn about:
- NGS platforms and NGS data files and formats
- NGS bioinformatics workflow steps following sequence generation
- Experimental NGS design and planning for your bioinformatics needs
- NGS for genomics; assembly, alignment, QC and variant calling tools
- NGS for transcriptomics; QC, mapping, visualisation tools
- EMBL-EBI resources: Array Express and Expression Atlas, ENA and the CRAM toolkit
After this course you should be able to:
- Discuss a variety of applications and workflow approaches for NGS technologies
- Use a range of bioinformatics software and tools to undertake basic analysis of NGS data
- Understand the advantages and limitations of NGS analyses
- Submit, browse and access a range of NGS data available in public repositories using EBI resources
**Registration is now closed
There are 30 places available on this course and we receive a high number of applications per space.
Places will be offered following an application process and selection of suitable candidates. Selection will be based on the strength of individuals’ applications, and potential benefits to science and research upon their return to host institutes. Where applicable, only a single applicant will be selected per institute. We also require a letter of support from a supervisor or senior colleague in addition to the completed form.
Items marked * are mandatory. Incomplete applications will not be processed.
The registration fee for this course is £495 and includes:
- All refreshments and lunches
- 3 breakfasts and 3 evening meals
- Accommodation for 3 nights at the Wellcome Trust Conference Centre
- Use of a computer in an EMBL-EBI training room
- Access to course materials following the course