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SAS results for UniProt accession no. P35751

Sequence annotated by structure

Key:
Sec. struc: By homology
  Helix Strand  
Residue contacts:   to ligand
Active sites:   (from PDB SITE records)

Secondary structure shown in red comes from a homologous protein structure which is at least 30% sequence identical to the target protein and has an alignment overlap of at least 80 residues or at least three-quarters of the length of the target sequence (whichever overlap is the larger).

Click on annotated residues to get source(s) of each annotation.

 

FASTA alignment for UniProt accession no. P35751 - coloured by no. of contacts to ligand

Below are the FASTA results from a search of the sequence of UniProt accession no. P35751 against all protein sequences in the PDB. Identical sequences are grouped together and represented by a single 'consensus' sequence (asterisked) onto which all relevant annotations are mapped. The number of sequences represented by each consensus sequence is shown by the '×n' on the right of the alignment. Use the box below to modify the annotations shown on the alignment. At the bottom of the page are given the FASTA stats for all the sequences. There you can exclude any sequences from the alignment.

Struct

FASTA

The search returned 48 unique sequences (including 5 consensus sequences) giving 56 sequence matches in all. The 32 sequences excluded from the alignment are listed at the end.

Modify alignment: Annotate by:
  Number:  
  Show secondary structure: Yes No  


                1         2         3         4         5         6     
       12345678901234567890123456789012345678901234567890123456789012345   Protein name
       ---------+---------+---------+---------+---------+---------+-----   ------------
P35751 -nkasvvanqlipintaltlimmkaevvtpmgipaeeipklvgmqvnravplgttlmpdmvknye   Ice-structuring protein RD1 OS=Rhigophila dearborni PE=1  ...
1ucs:A -nkasvvanqlipintaltlimmkaevvtpmgipaeeipklvgmqvnravplgttlmpdmvknye   Type iii antifreeze protein rd1 from an antarctic eel pout
3nla:A*-nkasvvanqlipintaltlimmkaevvtpmgipaeeipnlvgmqvnravplgttlmpdmvknye×2 Nmr structure of the n-terminal domain with a linker  ...
1c89:A*-nkasvvanqlipintaltlimmkaevvtpmgipaeeipnlvgmqvnravplgttlmpdmvknye×2 Nmr structure of intramolecular dimer antifreeze protein  ...
9ame:A anqasvvanqlipintaltlvmmrsevvtpvgipaediprlvgmqvnravplgttlmpdmvkgya   Type iii antifreeze protein isoform hplc 12 s42g
1gzi:A*-nqasvvanqlipintaltlvmmrsevvtpvgipaediprlvsmqvnravplgttlmpdmvkgya×3 Crystal structure of type iii antifreeze protein from ocean  ...
1ame:A anqasvvanqlipintaltlvmmrsevvtpvgipaediprlvsmqvnravplgttlmpdmvkgya   Crystal structure of type iii antifreeze protein at 4 c
1hg7:A MNQASVVANQLIPINTALTLVMMRSEVVTPVGIPAEDIPRLVSMQVNRAVPLGTTLMPDMVKGYA   High resolution structure of hplc-12 type iii antifreeze  ...
1b7i:A anqasvvanqlipintaltlvmmrsevvtpvgipaediprlvsmqvnravplgttlmpdmvrgya   Type iii antifreeze protein isoform hplc 12 k61r
1jab:A anqasvvanqlipintalslvmmrsevvtpvgipaediprlvsmqvnravplgttlmpdmvkgya   Type iii antifreeze protein isoform hplc 12 t18s
2spg:A anqasvvanqlipinsaltlvmmrsevvtpvgipaediprlvsmqvnravplgttlmpdmvkgya   Type iii antifreeze protein isoform hplc 12 t15s
3qf6:A -NQASVVANQLIPINTALTLVMMRSEVVTPVGIPADDIPRLVSMQVNRAVPLGTTLMPDMVKGYP   Neutron structure of type-iii antifreeze protein allows the  ...
1b7j:A anqasvvanqlipintaltlammrsevvtpvgipaediprlvsmqvnravplgttlmpdmvkgya   Type iii antifreeze protein isoform hplc 12 v20a
1ekl:A anqasvvanqlipintaltlvmmrsevvtpvgipakdiprlvsmqvnravplgttlmpdmvkgya   Type iii antifreeze protein isoform hplc 12 e35k
1msj:A anqasvvanqlipinvaltlvmmrsevvtpvgipaediprlvsmqvnravplgttlmpdmvkgya   Type iii antifreeze protein isoform hplc 12 t15v
4ame:A anqasvvanqlipintalalvmmrsevvtpvgipaediprlvsmqvnravplgttlmpdmvkgya   Type iii antifreeze protein isoform hplc 12 t18a
7ame:A anqasvvanqlipinaaltlvmmrsevvtpvgipaediprlvsmqvnravplgttlmpdmvkgya   Type iii antifreeze protein isoform hplc 12 t15a
9msi:A anqasvvanqlipintalnlvmmrsevvtpvgipaediprlvsmqvnravplgttlmpdmvkgya   Type iii antifreeze protein isoform hplc 12 t18n
2msj:A anqasvvanqlipintaltlvmmrsevvtpvgipaediprlvsmqvsravplgttlmpdmvkgya   Type iii antifreeze protein isoform hplc 12 n46s
1b7k:A anqasvvanqlipintaltlvmmrsevvtpvgipaediprlvsmqvnhavplgttlmpdmvkgya   Type iii antifreeze protein isoform hplc 12 r47h
1msi:A aaqasvvanqlipintaltlvmmrsevvtpvgipaediprlvsmqvnravplgttlmpdmvkgya   Structure of antifreeze glycoprotein qae(hplc 12)

                                         1         1         1         1
           7         8         9         0         1         2         3
       67890123456789012345678901234567890123456789012345678901234567890   Protein name
       ----+---------+---------+---------+---------+---------+---------+   ------------
P35751 -----------------------------------------------------------------   Ice-structuring protein RD1 OS=Rhigophila dearborni PE=1  ...
1ucs:A -----------------------------------------------------------------   Type iii antifreeze protein rd1 from an antarctic eel pout
3nla:A*dgttspglk--------------------------------------------------------×2 Nmr structure of the n-terminal domain with a linker  ...
1c89:A*dgttspglksvvanqlipintaltlvmmkaeevspkgipseeisklvgmqvnravyldqtlmpdm×2 Nmr structure of intramolecular dimer antifreeze protein  ...
9ame:A a----------------------------------------------------------------   Type iii antifreeze protein isoform hplc 12 s42g
1gzi:A*a----------------------------------------------------------------×3 Crystal structure of type iii antifreeze protein from ocean  ...
1ame:A a----------------------------------------------------------------   Crystal structure of type iii antifreeze protein at 4 c
1hg7:A A----------------------------------------------------------------   High resolution structure of hplc-12 type iii antifreeze  ...
1b7i:A a----------------------------------------------------------------   Type iii antifreeze protein isoform hplc 12 k61r
1jab:A a----------------------------------------------------------------   Type iii antifreeze protein isoform hplc 12 t18s
2spg:A a----------------------------------------------------------------   Type iii antifreeze protein isoform hplc 12 t15s
3qf6:A P----------------------------------------------------------------   Neutron structure of type-iii antifreeze protein allows the  ...
1b7j:A a----------------------------------------------------------------   Type iii antifreeze protein isoform hplc 12 v20a
1ekl:A a----------------------------------------------------------------   Type iii antifreeze protein isoform hplc 12 e35k
1msj:A a----------------------------------------------------------------   Type iii antifreeze protein isoform hplc 12 t15v
4ame:A a----------------------------------------------------------------   Type iii antifreeze protein isoform hplc 12 t18a
7ame:A a----------------------------------------------------------------   Type iii antifreeze protein isoform hplc 12 t15a
9msi:A a----------------------------------------------------------------   Type iii antifreeze protein isoform hplc 12 t18n
2msj:A a----------------------------------------------------------------   Type iii antifreeze protein isoform hplc 12 n46s
1b7k:A a----------------------------------------------------------------   Type iii antifreeze protein isoform hplc 12 r47h
1msi:A a----------------------------------------------------------------   Structure of antifreeze glycoprotein qae(hplc 12)

            
            
       12345                                                               Protein name
       -----                                                               ------------
P35751 -----                                                               Ice-structuring protein RD1 OS=Rhigophila dearborni PE=1  ...
1ucs:A -----                                                               Type iii antifreeze protein rd1 from an antarctic eel pout
3nla:A*-----                                                            ×2 Nmr structure of the n-terminal domain with a linker  ...
1c89:A*vknye                                                            ×2 Nmr structure of intramolecular dimer antifreeze protein  ...
9ame:A -----                                                               Type iii antifreeze protein isoform hplc 12 s42g
1gzi:A*-----                                                            ×3 Crystal structure of type iii antifreeze protein from ocean  ...
1ame:A -----                                                               Crystal structure of type iii antifreeze protein at 4 c
1hg7:A -----                                                               High resolution structure of hplc-12 type iii antifreeze  ...
1b7i:A -----                                                               Type iii antifreeze protein isoform hplc 12 k61r
1jab:A -----                                                               Type iii antifreeze protein isoform hplc 12 t18s
2spg:A -----                                                               Type iii antifreeze protein isoform hplc 12 t15s
3qf6:A -----                                                               Neutron structure of type-iii antifreeze protein allows the  ...
1b7j:A -----                                                               Type iii antifreeze protein isoform hplc 12 v20a
1ekl:A -----                                                               Type iii antifreeze protein isoform hplc 12 e35k
1msj:A -----                                                               Type iii antifreeze protein isoform hplc 12 t15v
4ame:A -----                                                               Type iii antifreeze protein isoform hplc 12 t18a
7ame:A -----                                                               Type iii antifreeze protein isoform hplc 12 t15a
9msi:A -----                                                               Type iii antifreeze protein isoform hplc 12 t18n
2msj:A -----                                                               Type iii antifreeze protein isoform hplc 12 n46s
1b7k:A -----                                                               Type iii antifreeze protein isoform hplc 12 r47h
1msi:A -----                                                               Structure of antifreeze glycoprotein qae(hplc 12)

Residue colours: black = 0, purple = 1, blue = 2, green = 3, orange = 4, red = 5 or more contacts.

Sequence listing

The listing below shows the FASTA statistics for the PDB entries that matched UniProt accession no. P35751. Ditto marks indicate identical sequences represented by a single representative sequence in the alignment above. The representative is asterisked and the names of all its duplicate sequences are indented to the right.

Structures whose names are given in purple are those that are annotated above. The parameters defining which sequences can transfer their annotations are shown at the bottom of the page where they can be altered and the alignment regenerated.

Use the checkboxes to the left of the PDB codes to exclude any sequences from the alignment (or to add back any sequences in the Excluded list). Then click the SELECT button at the bottom of the page to redisplay the alignment.

Aligned sequences

  Smith-
Waterman
score
%-tage
identity
a.a.
overlap
Seq
len
z-score E-
value
  PDB
code
Protein name
1. - - - 64 - - P35751 Ice-structuring protein RD1 OS=Rhigophila dearborni PE=1 SV=1
2. 401 100.0% 64 64 604.8 1e-26 1ucs:A Type iii antifreeze protein rd1 from an antarctic eel pout
3. 395 98.4% 64 73 595.0 3.6e-26 3nla:A * Nmr structure of the n-terminal domain with a linker portion of antarctic eel pout antifreeze protein rd3, 40 structures
4. " " " " " "  = 3rdn:A *
Nmr structure of the n-terminal domain with a linker portion of antarctic eel pout antifreeze protein rd3, minimized average structure
5. 395 98.4% 64 134 590.5 6.5e-26 1c89:A * Nmr structure of intramolecular dimer antifreeze protein rd3, 40 sa structures
6. " " " " " "  = 1c8a:A *
Nmr structure of intramolecular dimer antifreeze protein rd3, 40 sa structures
7. 363 87.3% 63 66 548.9 1.3e-23 9ame:A Type iii antifreeze protein isoform hplc 12 s42g
8. 355 85.7% 63 65 537.3 5.9e-23 1gzi:A * Crystal structure of type iii antifreeze protein from ocean pout, at 1.8 angstrom resolution
9. " " " " " "  = 1kde:A *
North-atlantic ocean pout antifreeze protein type iii isoform hplc12 mutant, nmr, 22 structures
10. " " " " " "  = 1kdf:A *
North-atlantic ocean pout antifreeze protein type iii isoform hplc12 mutant, nmr, minimized average structure
11. 355 85.7% 63 66 537.2 6e-23 1ame:A Crystal structure of type iii antifreeze protein at 4 c
12. 355 85.7% 63 66 537.2 6e-23 1hg7:A High resolution structure of hplc-12 type iii antifreeze protein from ocean pout macrozoarces americanus
13. 352 84.1% 63 66 532.8 1.1e-22 1b7i:A Type iii antifreeze protein isoform hplc 12 k61r
14. 352 84.1% 63 66 532.8 1.1e-22 1jab:A Type iii antifreeze protein isoform hplc 12 t18s
15. 352 84.1% 63 66 532.8 1.1e-22 2spg:A Type iii antifreeze protein isoform hplc 12 t15s
16. 351 84.1% 63 65 531.5 1.3e-22 3qf6:A Neutron structure of type-iii antifreeze protein allows the reconstruction of afp-ice interface
17. 350 85.7% 63 66 529.9 1.5e-22 1b7j:A Type iii antifreeze protein isoform hplc 12 v20a
18. 350 84.1% 63 66 529.9 1.5e-22 1ekl:A Type iii antifreeze protein isoform hplc 12 e35k
19. 350 84.1% 63 66 529.9 1.5e-22 1msj:A Type iii antifreeze protein isoform hplc 12 t15v
20. 350 84.1% 63 66 529.9 1.5e-22 4ame:A Type iii antifreeze protein isoform hplc 12 t18a
21. 350 84.1% 63 66 529.9 1.5e-22 7ame:A Type iii antifreeze protein isoform hplc 12 t15a
22. 350 84.1% 63 66 529.9 1.5e-22 9msi:A Type iii antifreeze protein isoform hplc 12 t18n
23. 349 84.1% 63 66 528.4 1.9e-22 2msj:A Type iii antifreeze protein isoform hplc 12 n46s
24. 348 84.1% 63 66 526.9 2.2e-22 1b7k:A Type iii antifreeze protein isoform hplc 12 r47h
25. 348 85.5% 62 66 526.9 2.2e-22 1msi:A Structure of antifreeze glycoprotein qae(hplc 12)

Number of sequences: 25

Excluded sequences

  Smith-
Waterman
score
%-tage
identity
a.a.
overlap
Seq
len
z-score E-
value
  PDB
code
Protein name
1. 348 84.1% 63 66 526.9 2.2e-22 2ame:A Type iii antifreeze protein isoform hplc 12 n14q
2. 347 84.1% 63 66 525.5 2.7e-22 6ame:A Type iii antifreeze protein isoform hplc 12 m21a
3. 346 84.1% 63 66 524.0 3.3e-22 2jia:A Type iii antifreeze protein isoform hplc 12 k61i
4. 343 83.9% 62 66 519.6 5.7e-22 4msi:A Type iii antifreeze protein isoform hplc 12
5. 342 83.9% 62 66 518.2 6.9e-22 2msi:A Type iii antifreeze protein isoform hplc 12
6. 342 83.9% 62 66 518.2 6.9e-22 5msi:A Type iii antifreeze protein isoform hplc 12
7. 342 82.5% 63 66 518.2 6.9e-22 8ame:A Type iii antifreeze protein isoform hplc 12 n14sa16h
8. 341 83.9% 62 66 516.7 8.4e-22 3msi:A Type iii antifreeze protein isoform hplc 12
9. 341 83.9% 62 66 516.7 8.4e-22 7msi:A Type iii antifreeze protein isoform hplc 12
10. 341 82.5% 63 66 516.7 8.4e-22 8msi:A Type iii antifreeze protein isoform hplc 12 n14sq44t
11. 339 82.5% 63 66 513.8 1.2e-21 3ame:A Type iii antifreeze protein isoform hplc 12 q9tq44t
12. 337 83.9% 62 66 510.8 1.8e-21 6msi:A Type iii antifreeze protein isoform hplc 12
13. 311 73.0% 63 67 472.6 2.4e-19 2lx3:A 1h,13c,15n assignments for an isoform of the type iii antifr protein from notched-fin eelpout
14. 289 69.8% 63 67 440.4 1.5e-17 2lx2:A 1h,13c,15n assignments for an isoform of the type iii antifr protein from notched-fin eelpout
15. 228 61.7% 60 64 351.4 1.3e-12 1ops:A Ice-binding surface on a type iii antifreeze protein from ocean pout
16. 128 39.7% 63 79 203.4 0.00024 1wvo:A Solution structure of rsgi ruh-029, an antifreeze protein like domain in human n-acetylneuraminic acid phosphate synthase gene.
17. 71 32.6% 46 253 111.2 32 4gwp:E * Structure of the mediator head module from s. Cerevisiae
18. " " " " " "  = 4gwq:E *
Structure of the mediator head module from s. Cerevisiae in with the carboxy-terminal domain (ctd) of RNA polymerase ii subunit
19. 71 33.3% 42 258 111.0 33 3frn:A Crystal structure of flagellar protein flga from thermotoga msb8
20. 72 26.3% 57 335 110.6 35 4ij1:A Bianthranilate-like analogue bound to anthranilate phosphoribosyltransferase (anprt; trpd) in absence of subst
21. 72 26.3% 57 338 110.5 35 3uu1:A Anthranilate phosphoribosyltransferase (trpd) from mycobacte tuberculosis (complex with inhibitor acs142)
22. 72 26.3% 57 344 110.4 36 3r88:A Anthranilate phosphoribosyltransferase (trpd) from mycobacte tuberculosis (complex with inhibitor acs145)
23. 72 26.3% 57 346 110.3 36 1zvw:A The crystal structure of trpd (rv2192c) from mycobacterium tuberculosis in complex with prpp and magnesium
24. 72 26.3% 57 345 110.3 36 2bpq:A Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (apo structure)
25. 72 26.3% 57 346 110.3 36 3qqs:A Anthranilate phosphoribosyltransferase (trpd) from mycobacte tuberculosis (complex with inhibitor acs172)
26. 72 26.3% 57 347 110.3 36 3qr9:A Anthranilate phosphoribosyltransferase (trpd) from mycobacte tuberculosis (apo structure)
27. 72 26.3% 57 346 110.3 36 3qs8:A * Anthranilate phosphoribosyltransferase (trpd) from mycobacte tuberculosis (complex with inhibitor acs174)
28. " " " " " "  = 3qsa:A *
Anthranilate phosphoribosyltransferase (trpd) from mycobacte tuberculosis (complex with inhibitor tamu-a7)
29. " " " " " "  = 3r6c:A *
Anthranilate phosphoribosyltransferase (trpd) from mycobacte tuberculosis (complex with inhibitor acs179)
30. " " " " " "  = 3twp:A *
Crystal structure of m. Tuberculosis trpd in complex with an
31. 72 26.3% 57 346 110.3 36 4giu:A Bianthranilate-like analogue bound in inner site of anthrani phosphoribosyltransferase (anprt; trpd).
32. 72 26.3% 57 346 110.3 36 4gkm:A Bianthranilate-like analogue bound in the outer site of anth phosphoribosyltransferase (anprt; trpd)

Number of sequences: 32

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Annotation parameters

The parameters below were used in determining which structural annotations could be transferred on to the target sequence. You can change the parameters and regenerate the SAS alignments and annotation by pressing the Rerun button.

Min. seq. identity:   Min. seq. overlap   Max. E-value

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