Figure 4 - full size

 

Figure 4.
Figure 4. Comparison of the active-site structures in the activated RuBP complex (magenta), the non-activated RuBP complex (blue) and the 2CABP complex (atoms coloured according to atom type). In the open form of the enzyme (activated RuBP complex, magenta), loop 6 of the a/b-barrel is swung away from the active site, and lies outside the image frame of the Figure. In the closed structures, the loop is folded over the active site with Lys334 at its tip, interacting with the substrate. Further differences between the open and closed forms are seen in the N-terminal loops, one beginning with Glu60 and the other centred at Asn123. Coordinates for the 2CABP complex of spinach rubisco are from the 1.6 Å resolution refined structure (8RUC, Andersson, 1996). Superposition of the structures was carried out using O (Jones et al., 1991). The picture was produced with MOLSCRIPT (Kraulis, 1991), modified by Robert Esnouf (Oxford University, unpublished). For the sake of simplicity, only key residues are labelled. See the bottom stereo pair of Figure 5 (same orientation) for further residue numbering.

The above figure is reprinted by permission from Elsevier: J Mol Biol (1997, 265, 432-444) copyright 1997.