Figure 5. Surface Representations of T. maritima TF and S7
(A) Contact surfaces. The molecular surface of TF (middle)
is colored by domains as in 3B but with the imprint of bound S7
colored in yellow. The dark ribbon shows S7. Molecular surfaces
of S7 (outside) are rotated to display the imprint of bound TF.
Blue residues contact NTD, green contact PPIase, red contact
CTD, and magenta contact both NTD and CTD.
Electrostatic potential of TF and S7. Molecular surfaces are
oriented as in (A) and S7 is drawn as a yellow ribbon. Surfaces
are colored in degrees of positive (blue) and negative (red)
(C) Hydrophobic patches on the tmTF surface.
Vicinal apolar atoms that form continuous hydrophobic surfaces
are colored blue.
(D) Comparison of the TF:S7 interface
with approximately 44,000 structurally defined interfaces
between pairs of protein domains cataloged in PYBASE (Davis and
Sali, 2005). Properties of the TF:S7 interface are represented
by the red and blue spheres (1:1 dimer and 2:2 tetramer,
respectively). PYBASE interaction sets are represented by gray
spheres. Sc corresponds to shape complementarity value, BSA
corresponds to buried surface area, and P/NP corresponds to the
ratio of polar versus nonpolar interfacial residues.
The above figure is reprinted
by permission from Cell Press: