Figure 4 - full size

 

Figure 4.
Figure 4. Protein DNA Contacts in Wild-Type and N141A HincII/DNA Structures
(A) Stereo view of the contacts to the DNA by residues 138–141 of N141A HincII. Colored by atom type: N-blue, O-red, P-orange, C-green in protein, pink in DNA of the recognition site and yellow in flanking base pairs. A water molecule is shown as a red sphere. Dashes indicate hydrogen bonds.
(B) Stereo view of the contacts to the DNA by residues 138–141 of wild-type HincII. Colored as in (A). Dashes indicate hydrogen bonds.
(C) Stereo view of the contacts to the DNA at Thy 2′ and Gua 1′ of N141A HincII. Colored as in (A). Methyl groups are shown as green or pink spheres; water molecule is shown as a red sphere. Dashes indicate hydrogen bonds or van der Waals interactions.
(D) Stereo view of the contacts to the DNA at Thy 2′ and Gua 1′ of wild-type HincII. Colored as in (A). Methyl groups are shown as green or pink spheres. Dashes indicate hydrogen bonds or van der Waals interactions.
(E) Cartoon of protein-DNA contacts to bases in the major groove in the N141A HincII/DNA structure. Water molecule is shown as a red circle.
(F) Cartoon of protein-DNA contacts to bases in the major groove in the wild-type HincII/DNA structure. Dashes indicate hydrogen bonds or van der Waals interactions.

The above figure is reprinted from an Open Access publication published by Cell Press: Structure (2008, 16, 1828-1837) copyright 2008.