Proposed catalytic mechanism of hCAII. A schematic
representation of three discrete stages of the catalytic cycle
is shown. a, unbound. Note the presence of deep water (W[DW]).
b, CO bound. Note the displacement of W[DW] and the hydrogen
bond between the substrate and backbone amide of Thr-199. c, the
formation of bicarbonate. The figure was created using ChemDraw
11.0 (available from CambridgeSoft).
The above figure is reprinted
from an Open Access publication published by the ASBMB:
J Biol Chem