Figure 3 - full size


Figure 3.
FIGURE 3. Comparison of LC3 and its homologs. A, sequence alignments of human LC3, rat GABARAP, and bovine GATE-16. Identical amino acid residues are boxed. The secondary structural element of LC3 is indicated above the alignments. LC3 interacting residues of p62 peptide are indicated by red circles. B, interaction of p62M7 and LC3 homologs. MBP-p62M7 conjugated with amylose resins were incubated with purified LC3, GABARAP, and GATE-16. The pulled down products with MBP-p62M7 were detected in CBB-stained SDS-PAGE. C and D, models of the GATE-16-p62 peptide and GABARAP-p62 peptide complex from published structures (codes 1eo6 and 1kgt). GATE-16 and GABARAP are shown in electrostatic surface representation. p62 peptide is shown as a stick model. Residues that correspond to residues from LC3 shown in Fig. 2B are labeled.

The above figure is reprinted by permission from the ASBMB: J Biol Chem (2008, 283, 22847-22857) copyright 2008.