Figure 2 - full size

 

Figure 2.
FIGURE 2. Sedimentation-velocity analysis of the binding of MAM to HLA-DR1/HA. A, sedimentation coefficient distributions of MAM alone ( ), of DR1 alone ( ), and of mixtures at different ratio of concentrations as follows: MAM:DR1 = 6:6 µM ( ); = 30:10 µM ( ); and = 5:10 µM (•) in the absence of Zn^2+. B, sedimentation coefficient distributions of DR1 alone and MAM/DR1 mixtures under various conditions. , 8 µM DR1 with 1 mM EDTA; , 15 µM DR1 with 1 mM ZnCl[2]; , 6 µM MAM and 4 µM DR1 with 1 mM EDTA; , 6 µM MAM and 4 µM DR1 with 1 mM ZnCl[2]; •, 16 µM MAM and 1 µM DR1 with 1 mM ZnCl[2]; , 16 µM MAM and 1 µM DR1 with 1 mM ZnCl[2] and 10 mM EDTA.

The above figure is reprinted by permission from the ASBMB: J Biol Chem (2007, 282, 5991-6000) copyright 2007.