Figure 2 - full size


Figure 2.
Figure 2. Biochemical characterization of wild-type (WT) Ubc9 and mutants. (a) Initial rates of reaction versus p53 substrate concentration, fit to rectangular hyperbolas (Methods), are shown for wild-type Ubc9, Ubc9-N85Q, Ubc9-Y87A and Ubc9-D127A. Left and right y-axes indicate rates for wild-type and mutant enzymes, respectively. (b) Bar charts depicting the kinetic constants apparent K[d] and k[2] for wild-type and mutant Ubc9, plotted on a log scale (see Supplementary Table 1). Error bars in a and b are s.d.

The above figure is reprinted by permission from Macmillan Publishers Ltd: Nat Struct Mol Biol (2006, 13, 491-499) copyright 2006.