Figure 3 - full size

 

Figure 3.
Fig. 3. Characterization of the peptide binding site of PrsA-PPI. (A) Chemical shift changes observed upon binding of Suc-AKPF-pNA (black), Suc-AYPF-pNA (grey) and Suc-ANPF-pNA (blue). Changes at PrsA-PPI to peptide molar ratio 1:16 are shown. (B) Residues significantly perturbed upon binding are highlighted on PrsA-PPI structure. Red codes for large (0.07 slant Δδ < 0.36 ppm), and yellow for smaller (0.04 slant Δδ < 0.07 ppm) chemical shift changes. Backbone atoms of hPin1 (PDB ID 1pin) and PrsA-PPI were overlaid and the Ala-Pro dipeptide present in hPin1 structure is shown in blue. (C) A molecular surface representations of PrsA-PPI coloured according to its electrostatic potential. Negative residues are coloured in red, and positive in blue.

The above figure is reprinted by permission from the Federation of European Biochemical Societies: FEBS Lett (2006, 580, 1822-1826) copyright 2006.