Figure 1 - full size

 

Figure 1.
Figure 1 Crystal structures of wild-type MutS and E38 variants. (A) Schematic view of the E. coli MutS dimer, with the mismatch-binding monomer A shown in yellow, the supporting monomer B shown in orange and the mismatch containing DNA in blue. (B) Superposition of the mismatch-binding clamp domains and DNA of wild-type and mutant MutS obtained by superposing the C atoms of the mismatch-binding monomer A of all the structures. (C) G.T mismatch binding by the wild-type MutS. (D) G.T mismatch binding by the E38T mutant, with the bound water molecule in red, along with its mF[o]-DF[c] map contoured at 3 (in green). (E) G.T mismatch binding by the E38Q mutant. (F) G.T mismatch binding by E38A, shown with the bound water molecule (in red) along with its mF[o]-DF[c] density map contoured at 3 (in green). Dashed red lines indicate hydrogen bonds and residue 38 in panels C-F is shown in purple. All figures were made using PyMOL (Copyrightę 2004 DeLano Scientific).

The above figure is reprinted by permission from Macmillan Publishers Ltd: EMBO J (2006, 25, 409-419) copyright 2006.