Figure 2 - full size


Figure 2.
Figure 2: Properties of the RNase A amyloid-like fibrils. The hinge-loop region of wild-type RNase A that connects the C-terminal -strand (triangle in the diagrams in column 1) to the protein core is expanded with the -GQ[10]G- motif to generate amyloid-forming RNase A mutants. Two inactive RNase A mutants are formed by replacing His 12 or His 119 with Ala. Wild-type RNase A does not form fibrils and has a fully functional active site (row 1, columns 2 and 4). The Q[10]-H12A, Q[10]-H119A and Q[10]-H12A + Q[10]-H119A constructs all form amyloid-like fibrils (column 2) and bind Congo red with the characteristic apple-green birefringence (column 3). The Q[10]-H12A + Q[10]-H119A fibrils (row 4, column 4) have significantly higher activity than fibrils of Q[10]-H12A (row 2, column 4) and Q[10]-H119A (row 3, column 4) alone. This is a result of complementation of active sites by domain swapping. The expected activity of each of the constructs is given in parentheses. Scale bar (column 2), 200 nm.

The above figure is reprinted by permission from Macmillan Publishers Ltd: Nature (2005, 437, 266-269) copyright 2005.