Figure 4 - full size

 

Figure 4.
Figure 4. The pH profile of the logarithm of k[cat]/K[m] (M^-1s^-1) for hydration of CO[2] catalyzed by H64A HCA II ( ), and H64A-T200H HCA II ( ). Experimental conditions were as described in Figure 1. For H64A-T200H HCA II the solid line is a fit to two ionizations (Equation 6) with pK[a1] = 5.8 ± 0.4 and pK[a2] = 8.0 ± 0.4. For H64A HCA II the data are fit to a single ionization with pK[a] = 6.9 ± 0.1.

The above figure is reprinted by permission from John Wiley & Sons, Inc.: Proteins (2005, 61, 239-245) copyright 2005.