Figure 3 - full size

 

Figure 3.
Fig. 3. Electrostatic properties and effect of salt on enzymatic activity of mCA XIV. (a) The surface electrostatic potentials, the decrease in electrostatic free energy on transfer from 3.0 to 0.1 M NaCl, and surface density of acidic and basic residues are as described in Fig. 2. (b and c) Assays of CO[2] hydration activity (b) and ester hydrolysis activity (c) of dCA II (green), mCA XIV (yellow), or hCA I (red) were performed as described in Materials and Methods. Controls indicate enzyme activity in the absence of salt. For esterase activity, 0.5 mM p-nitrophenyl acetate was used as substrate. Pot, electrostatic potential.