Figure 1 - full size

 

Figure 1.
Figure 1. The P10 Position of Peptides Can Affect Binding to HLA-DR1(A) Surface of the HLA-DR1 peptide binding site with bound AAYSDQATPLLLSPR peptide shown as a stick model. In the peptide, carbon atoms are yellow, nitrogen atoms are blue, and oxygen atoms are red. Peptide side chain binding pockets are labeled.(B) Competitive binding analysis of two peptides that differ only in the P10 position. A fixed concentration of peptide-free HLA-DR1 and biotinylated Ha peptide was incubated with increasing concentrations of either AAYSDQATPLLLSPR or AAYSDQATPLLGSPR peptide. After binding for 3 days at 37°C, the amount of biotinylated Ha peptide/HLA-DR1 complexes was measured with alkaline phosphatase-labeled streptavidin in an antibody-capture assay.

The above figure is reprinted by permission from Cell Press: Chem Biol (2004, 11, 1395-1402) copyright 2004.