Figure 2 - full size

 

Figure 2.
Figure 2. (a) Geometry around the two main manganese ions. (b) Simulated annealing omit map at the 3s level of the HincII/DNA/Mn 2C structure subunit A where the nucleotides around the scissile bond, Cyt7 and Gua8, have been excluded. The manganese ions are shown as green spheres. (c) Simulated annealing omit map at the 3s level of the HincII/DNA/Mg 2C structure subunit A where the nucleotides around the scissile bond, Cyt7 and Gua8, have been excluded. Water molecules are shown as small red spheres. (d) Superposition of subunit A of HincII/cleaved DNA/Mn 2C (red) and HincII/cleaved DNA/Mg 2C (green) showing an equivalent position of the phosphate resulting from cleavage. Although both of the two main Mn 2C positions (large red sphere) were present in the Figure, only one is visible, as the view is down the Mn 2C --Mn 2C axis. Water molecules are indicated by small spheres.

The above figure is reprinted by permission from Elsevier: J Mol Biol (2004, 343, 833-849) copyright 2004.