Figure 2 - full size

 

Figure 2.
FIG. 2. Structure of the oxidized CLIC1 dimer. A, stereo backbone of the CLIC1 dimer; green, A subunit; red, B subunit. In the A subunit every 10th residue is labeled. B, electron density of the intramolecular disulfide bond between Cys-24 and Cys-59 contoured at 1 . The CLIC1 dimer is viewed along (C) and perpendicular (D) to the pseudo 2-fold axis; are shown helices, A subunit (red) and B subunit (green), and intramolecular disulfide bonds (yellow). E, ClustalW (24) alignment of the CLIC family. The secondary structure is shown for both monomeric (red, helices; yellow, -strands) and dimeric (blue, helices) forms. Conserved regions are shaded; green, putative transmembrane regions; yellow, Cys; cream, Gly. Features unique to CLIC1 are in blue. Ramachandran distances (see "Experimental Procedures") for the monomer to dimer transition are plotted above its sequence. Figures were made with SETOR (25), MOLSCRIPT (26), RASTER3D (27), and CONSCRIPT (28).

The above figure is reprinted by permission from the ASBMB: J Biol Chem (2004, 279, 9298-9305) copyright 2004.