Figure 1 - full size


Figure 1.
FIG. 1. COX substrate binding modes. Stereo diagram of the inhibitory binding mode of arachidonic acid to COX-2 (blue) aligned with the productive binding mode, observed in COX-1 (beige). The substrate is nominally rotated 180° between the two orientations, resulting in different coordination of the carboxylic acid group. Amino acids shown are within van der Waals contact of diclofenac except for Arg-120, Val-434, and Arg-513, added for reference. Red text indicates the position of the three amino acid differences between COX-1 and COX-2 within the active site. The superposition was performed using amino acids 113-122, 344-355, 385-391, and 522-532 and coordinate Protein Data Bank files 1CVU [PDB] and 1DIY [PDB] . All molecular graphics were generated with Ribbons and rendered with POV-Ray (36).

The above figure is reprinted by permission from the ASBMB: J Biol Chem (2003, 278, 45763-45769) copyright 2003.