Figure 2 - full size


Figure 2.
Fig. 2. Views of the three VHHs superimposed on PPA with the acarbose molecule bound (pink). The PPA surface is in gray, and its visible catalytic residue Asp-300 is in red. The C trace of the VHHs is orange, the three CDRs (1-3) are colored red, green, and blue, respectively, and each VHH surface is transparent. Inset, close-up view of the PPA active site with the inhibitory AMD9 VHH bound. The saccharidic inhibitor acarbose has been positioned in the active site according to the x-ray structure as a probe of the saccharide position. Two residues of the VHH, Tyr-52 and Arg-52a (CPK yellow and orange), clash with the modeled acarbose (views made with SPOCK (33)).

The above figure is reprinted by permission from the ASBMB: J Biol Chem (2002, 277, 23645-23650) copyright 2002.