Figure 1 - full size

 

Figure 1.
Fig. 1. EPA bound in the cyclooxygenase active site of the Co3+-oPGHS-1·EPA complex. A, stereo view of EPA bound in the COX channel. The initial positive F[o] F[c] electron density (purple) within the COX channel, contoured at 2.0 , is shown with the final refined model of EPA (light blue). Side chain atoms for residues that predominantly contact the substrate at the carboxylate, C-2 through C-14, and C-15 through C-20 are colored blue, red, and green, respectively. Ser-530 (purple) lies below Tyr-385 (orange). The side chain of Ser-353 has been omitted for clarity. Single-letter amino acid codes are used throughout figures. B, a view of the cyclooxygenase active site rotated 90° about the vertical axis using the same color scheme as in A. The positive F[o] F[c] "omit" electron density (red) contoured at 2.0 for EPA is shown. All figures were created fully or in part using the program SETOR (68).

The above figure is reprinted by permission from the ASBMB: J Biol Chem (2001, 276, 37547-37555) copyright 2001.