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Figure 9.
Figure 9. Schematic representation showing the change in
the relative orientation of the two CCHC and CCCC zinc-binding
sites in CRP2(LIM2)R122A introduced by the point mutation at
residue position 122. (a) Ribbon drawing showing the
superposition of a representative structure taken from the
ensemble of 11 energy-minimized NMR structures of
CRP2(LIM2)R122A (yellow) and the wild-type CRP2(LIM2) structure
with the lowest residual restraint violations [Konrat et al
1997] (blue). Backbone atoms of the N-terminal CCHC zinc finger
encompassing residues Cys120-Cys144 were matched. (b) Orthogonal
view displaying only the C-terminal CCCC zinc binding site of
mutant CRP2(LIM2)R122A (yellow) and wild-type protein (blue).
The pictures were produced with the program MOLMOL [Koradi et al
1996].
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