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Figure 8.
Figure 8 Changes in the solvent-accessible surface (a) for the
N1/N2 interface in the lactoferrin N-lobe and (b) for the
N-lobe/C-lobe interface. In each case the open ApoLf structure
is on the left and the closed Fe[2]Lf structure is on the right.
Surface area buried between N1 and N2 domains is much larger in
the closed form (a, right panel). On the other hand, the surface
area buried between the N- and C-lobes is greater in the open
form (b, left panel). In (a) the N2 domain surface is white and
the N1 domain surface is magenta; only the portions of the
surface that come into contact (within 4.5 Å) are shown.
The much larger contact surface for the closed form (a, right)
arises because the N2 domain (upper) rotates over the N1 domain
(lower) about an axis running left to right across the page. In
(b) the N-lobe surface is magenta and the C-lobe surface is
white. The greater contact area between the lobes in the open
form (left) arises because of the movement of the N2 domain
(upper, left) up against the connecting helix (H12) and the
C-lobe. The rotation axis is approximately about an axis running
top to bottom across the page. Figure prepared with GRASP
(Nicholls et al., 1993[Nicholls, A., Bharadwaj, R. & Honig, B.
(1993). Biophys. J. 64, 166.]).
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