|
Figure 7.
Role of Thr^67 on LC8-Pak1 interaction. A, stereoview of the
LC8-Pak1 hydrogen bond network. A conserved hydrogen bond
network in LC8 is formed through the side chains of Lys^43,
Asp^47, and Thr^67′ and the backbone of Trp^54 in LC8 and
interacts with Pak1 through the side chain of Asp^216 through a
short hydrogen bond (dotted red lines). B and C, analysis of
T67A mutant by analytical SEC. Analytical SEC assays demonstrate
that mutation of LC8-Thr^67 to alanine abrogates the Pak1-LC8
interaction. The dynein intermediate chain, on the other hand,
encodes threonine at the same position (Asp^216 in Pak1) and
does not form a significant hydrogen bond (distance is 5.54
Å based on Protein Data Bank entry 2PG1). Analytical SEC
assays show that the dynein IC binds to the mutant, T67A-LC8.
|
