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Figure 7.
Figure 7. The Inactive Assembled State of c-Src Is
Incompatible with the Csk:c-Src Complex
(A) The crystal
structure of inactive assembled c-Src (PDB code 2SRC) is aligned
on the C-terminal lobe of c-Src from our Csk[KD]:c-Src[KD]
structure. Clashes between the tail of inactive c-Src and the C
lobe of Csk are highlighted in black.
(B) Csk cannot bind
tail-phosphorylated c-Src as measured by surface plasmon
resonance. A construct of c-Src that contains the SH2, SH3, and
kinase domains (Src[3D]) was phosphorylated by Csk on Tyr 527
(Src[3D] pY527) prior to immobilization on the sensor surface.
The binding of Csk[FL] (10 μM) to the surface was then
measured. The graphs show the mean and standard deviation of two
experiments.
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