|
Figure 7.
Figure 7. Direct Interaction between Rpb1 His1085 and
α-Amanitin and TL Capture May Underlie α-Amanitin Inhibition
of Transcription
(A) Overall view of α-amanitin and the
new TL conformation and their positions in relation to the
Bridge helix (BH). A superpositioned EC structure (PDB 2E2H)
showing DNA (magenta), RNA (red), nontemplate DNA (green), and
nucleotide GTP (orange) highlights the position of the inhibitor
and TL in relation to EC components.
(B) A 90° rotation
shows the α-amanitin position in relation to the Bridge helix
(BH) and its capture of the TL Rpb1 His1085.
(C) TL
residues Rpb1 1084–1086 and the entire α-amanitin modeled
into electron density (dark gray mesh) from an initial unbiased
2Fo-Fc electron density map contoured at 0.6 σ.
|
