Figure 7.
FIGURE 7. In the crystal structure of the hairpin
deletion variant, all four molecules have DNA in the polymerase
active site. A and B, two of the four complexes found in the
asymmetric unit of the previously solved furan-dAMP binary
complex (PDB code 1RV2 (15)). One complex has DNA in the
exonuclease active center (molecule B) (A); the other has DNA in
the polymerase active site (molecule C) (B). C and D, the
equivalent complexes from the structure of the -variant
inserting an A opposite furan. In this structure none of the
four complexes within the asymmetric unit has DNA in the
exonuclease (exo) domain. The polymerase domains are colored red
for the palm, green for the thumb, blue for the fingers, cyan
for the exonuclease (with the hairpin in black), and
orange for the N-terminal domain. The primer strand is shown in
magenta and the template in dark blue. The tip of the hairpin
loop was disordered in A.
The above figure is reprinted
by permission from the ASBMB:
J Biol Chem
(2007,
282,
1432-1444)
copyright 2007.
hairpin
deletion variant, all four molecules have DNA in the polymerase
active site. A and B, two of the four complexes found in the
asymmetric unit of the previously solved furan-dAMP binary
complex (PDB code 1RV2 (15)). One complex has DNA in the
exonuclease active center (molecule B) (A); the other has DNA in
the polymerase active site (molecule C) (B). C and D, the
equivalent complexes from the structure of the