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Figure 7.
Figure 7 Molecular model for the interaction of Mud1 UBA with
K48-linked Ub[2]. (A) Intensity changes by cross-saturation of
the 15N -1H cross-peaks in 2H, 15N-Mud1 UBA in complex with
unlabeled K48-Ub[2]. (B) Primary (left) and secondary (right)
binding sites on Mud1UBA as identified by NMR cross-saturation.
Resonances showing intensity ratios <0.5 or 0.3 are displayed on
the molecular surface of Mud1 UBA in light or dark red,
respectively. (C) Closed conformation of Ub[2], based on the
crystal structure obtained under basic conditions (PDB accession
code 1AAR) (Cook et al, 1992). The hydrophobic patches on each
Ub moiety interact with each other. The proximal and distal
moieties of Ub[2] are colored in red and blue, respectively. (D)
Open conformation of Ub[2], in equilibrium with closed
conformation in solution. The two hydrophobic clusters formed by
residues Leu8, Ile44, His68 and Val70 are available for binding
of a single UBA domain via a primary (purple) and a secondary
(blue) Ub-binding sites.
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