Figure 7.
FIG. 7. Proposed antibiotic resistance mechanism. Step ,
this is from the native drNimA structure to the covalently bound
pyruvate structure (drNimA-Pyr), an oxidation of His-71 and
pyruvate into a His-71-Pyr residue, a reaction that releases
2e^- and H+. Step ,
the released electrons can further be used to reduce the
antibiotic. Because the antibiotic gets 2e^-, it prevents
formation of the toxic bactericidal radical as
given in Fig. 1. Our drNimA-MTR structure seems to be an
intermediate, which is located somewhere along Step in
between the native drNimA and the drNimA-Pyr complex.
The above figure is reprinted
by permission from the ASBMB:
J Biol Chem
(2004,
279,
55840-55849)
copyright 2004.
,
this is from the native drNimA structure to the covalently bound
pyruvate structure (drNimA-Pyr), an oxidation of His-71 and
pyruvate into a His-71-Pyr residue, a reaction that releases
2e^- and H+. Step 
,
the released electrons can further be used to reduce the
antibiotic. Because the antibiotic gets 2e^-, it prevents
formation of the toxic bactericidal radical 
as
given in Fig. 1. Our drNimA-MTR structure seems to be an
intermediate, which is located somewhere along Step