|
Figure 6.
Figure 6. Comparison of X-ray and NMR structures of the
individual ninth and tenth modules of human and mouse
FnFn3(9,10). (a) The ensemble of 20 accepted NMR structures,
with the core residues of the ninth and tenth modules used to
independently align the respective coordinates of the individual
modules. (b) MolMol [Koradi et al 1996] ribbon drawing of the
average NMR structure of the ninth module (upper) and the tenth
module (lower). The average NMR structure of the ninth module
(upper) was calculated by aligning the coordinates of its core
residues, and the average NMR structure of the tenth module
(lower) was calculated in analogous fashion; the diagonal double
bars between the upper (ninth) and lower (tenth) module
structures in (a) and (b) are to emphasize that the alignments
were carried out separately for the two modules. (c) MolMol
[Koradi et al 1996] ribbon drawing of the X-ray structure of
hFnFn3(9,10) illustrating the homologous structures of the ninth
(upper) and tenth (lower) modules. (d), (e) Comparison of Δφ
and Δ  ,
the differences between the NMR and X-ray φ, (d), and ,
(e), angles. The NMR φ, angles
are the average φ, angles
calculated from the 20 accepted NMR structures. In general the
values of Δφ and Δ are
inversely correlated with the number of NOE restraints per
residue (Figure 8(a).
|
