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Figure 6.
Figure 6. Electrostatic potential surface representation of
the model of tryptase. (a) The region around the active site,
including the binding loop of LDTI. (b) The same view as (a) but
rotated 180°. The sidechains of the specificity residues P4 to
P3' of LDTI are labeled (nomenclature of Berger and Schechter
[26]), although Ala5 (P4) is hidden under the tryptase 174
insertion loop and Lys8 (P1) is buried in the S1 specificity
pocket. The highly electronegative active-site area (red)
corresponds well to the positively charged LDTI molecule (pI >
10). The concentrated positively charged region (blue) in
tryptase, shown at the bottom of (b), could be responsible for
heparin binding. (The figure was made with the program GRASP
[43].)
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