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Figure 6.
Figure 6. Crystal Structure of Ubc9^*SUMO
(A) The
Ubc9^*SUMO structure. Covalent linkage between Ubc9 Lys14 and
the SUMO C terminus.
(B) The interface between Ubc9 and
SUMO (H bonds: gray dotted lines, interface side chains as
sticks).
(C) Superposition of Ubc9^*SUMO and E2-25K^*SUMO.
(D) Buried surface area between E2 and SUMO plotted against
the sequences of Ubc9 and E2-25K.
(E) Model of the
Ubc9^Δhairpin mutant. Deleted β-hairpin is in gray.
(F)
Thioester formation for Ubc9^Δhairpin (670 nM) versus
Ubc9^Δhairpin*SUMO (670 nM) assay as in Figure 3, with 260 nM
Aos1-Uba2, 6.7 μM SUMO, and immunoblotting with α-Ubc9. Colors
are as follows: blue Ubc9 (yellow catalytic Cys) with yellow
SUMO, cyan E2-25K with pink SUMO.
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