Figure 6 - full size

 

Figure 6.
The TIR1/AFB specific probe 8 blocks auxin responses of moss P. patens. (A) Effects of 8 on NAA-induced elongation of P. patens gametophores. The juvenile gametophore was incubated for 60 h with chemicals (2 μM NAA and/or 20 μM 8). Arrows indicate the elongation zone in response to NAA. (Scale bar, 10 mm.) (B) Effects of 8 and NAA on the development of chloronemata. Chloronema cells were cultured on a BCDATG medium for 10 days in the presence of 0.5 μM NAA and/or 10 μM 8. Arrows indicate caulonemata.