Figure 6.
Figure 6 Structure of Rna1p refined to 2.20 Å. (a)
Representative view of the final 3F[o] - 2F[c] electron-density
map. Shown is the region around leucine-rich repeat 8 (LRR8),
contoured at 1.5 .
(b) Ribbon representation of Rna1p. (c) Superimposition of the
two independent molecules A (red) and B (blue) in the asymmetric
unit (C^ backbone
representation). Differences are found in the N-terminal region
as well as in LRR3/LRR4. (d) Superimposition of Rna1p (molecules
A and B in red and blue, respectively) and the complex of Rna1p
(green) with Ran-GMPPNP-Mg-RanBP1 (PDB code 1k5d ). The Ran
backbone is shown as a grey ribbon, GMPPNP in stick
representation and Mg as magenta-coloured sphere. An enlargement
of the region of the flexible loops of LRR3/LRR4 is shown. This
region, which differs between molecules A and B in the
high-resolution structure of Rna1p, coincides with part of the
interface between Rna1p and Ran-GMPPNP. The flexibility in Rna1p
may indicate an inherent mobility designed to allow an induced
fit.
The above figure is reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(2006,
62,
750-765)
copyright 2006.
.
(b) Ribbon representation of Rna1p. (c) Superimposition of the
two independent molecules A (red) and B (blue) in the asymmetric
unit (C^ 
backbone
representation). Differences are found in the N-terminal region
as well as in LRR3/LRR4. (d) Superimposition of Rna1p (molecules
A and B in red and blue, respectively) and the complex of Rna1p
(green) with Ran-GMPPNP-Mg-RanBP1 (PDB code 1k5d ). The Ran
backbone is shown as a grey ribbon, GMPPNP in stick
representation and Mg as magenta-coloured sphere. An enlargement
of the region of the flexible loops of LRR3/LRR4 is shown. This
region, which differs between molecules A and B in the
high-resolution structure of Rna1p, coincides with part of the
interface between Rna1p and Ran-GMPPNP. The flexibility in Rna1p
may indicate an inherent mobility designed to allow an induced
fit.