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Figure 6.
Figure 6. Identification of DNA-binding site. (a) Sequence
variation with DNA binding induced chemical shift changes, Click
to view the MathML source- [0?wchp=dGLbVtb-zSkWA]
, of the AtTRP1[464-560] amide resonances, where Dd[NH] and
Dd[N] is the chemical-shift difference for NH and 15N,
respectively. (b) A schematic representation of the
AtTRP1[464-560] structure. The red rods represent the helices.
(c) Electrostatic charge potential on the surface of
AtTRP1[464-560]. (d) The spatial locations of the DNA-perturbed
residues of AtTRP1[464-560]. The magnitudes of the DNA
binding-induced chemical shifts are color-coded as follow: red,
Dd>0.6 ppm; orange, 0.6 >Dd> 0.4 ppm; yellow, 0.4>Dd>0.2 ppm;
white, 0.02>Dd>0 ppm. Proline residues, or residues with amide
resonances that cannot be observed, are colored dark grey. All
structures are shown in the same orientation as in (b). (e) A
model structure of AtTRP1[464-560] bound to a double-stranded
telomeric DNA (sequence 5'-TAGGGTTTAGGGT-3'). The backbone of
the protein molecule is shown as a yellow rope and the four
helices are shown as red cylinders. The green sticks indicate
residues with significant chemical shift perturbations attained
upon binding to DNA. Grey and blue sticks represent the
backbones of the two strands of DNA molecules, and the
negatively charged phosphate group, respectively.
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