Figure 6.
Figure 6. Correlation of the autoinhibited conformation of X11
with
the target-binding property of the protein. The C termini of
presenilin (DQLAFHQFYI) and calcium channel (HHPDQDHWC) were
fused to glutathione S-transferase, respectively. Purified
recombinant GST fusion proteins were used for binding assays
with wild-type (WT) X11 and
its various mutants, including Y836E, Y836F, PDZ1^* (PDZ1
deficient in ligand binding), PDZ2^* (PDZ2 deficient in ligand
binding) and PDZ12^* (PDZ2 with both its ligand binding sites
disrupted). The amount of X11 protein
was detected by immunoblotting with antibody to c-Myc (9E10).
The above figure is reprinted
by permission from Macmillan Publishers Ltd:
Nat Struct Mol Biol
(2005,
12,
722-728)
copyright 2005.
with
the target-binding property of the protein. The C termini of
presenilin (DQLAFHQFYI) and calcium channel (HHPDQDHWC) were
fused to glutathione S-transferase, respectively. Purified
recombinant GST fusion proteins were used for binding assays
with wild-type (WT) X11 
and
its various mutants, including Y836E, Y836F, PDZ1^* (PDZ1
deficient in ligand binding), PDZ2^* (PDZ2 deficient in ligand
binding) and PDZ12^* (PDZ2 with both its ligand binding sites
disrupted). The amount of X11