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Figure 6.
Figure 6. Stereo-views of heavy chain residues 93-102 from
the two ligand-free structures, which includes HCDR3. For
reference, the sequence of heavy chain residues 93-102 is
93VRGGYRP100Y100AY100BA^100CMD^102Y. (a) HCDR3 from Fab 2 of the
P6[5] structure. (b) HCDR3 from Fab 1 of the P321 structure. The
electron density maps for both panels are simulated annealing
s[A]-weighted mF[o] -DF[c] maps and the contour level is 3s.
Prior to map calculation, heavy chain residues 94-101, along
with surrounding residues within 3.9 Å, were omitted from
the model and simulated annealing was performed. Note that
residues 98-100A (RPYY) are disordered, and they appear in the
deposited PDB entries with occupancy of zero. A theoretical
model of the backbone of residues 98-100A is drawn to help guide
the eye.
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