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Figure 6.
Figure 6. Activation of ERK MAP Kinase by SOSCOS1 cells
were transiently cotransfected with HA-tagged ERK2 and T7-tagged
SOS constructs as indicated. ERK2 activation was measured in
serum-starved cells by an immunoprecipitated kinase-kinase assay
using myelin basic protein (MBP) as a substrate. Results were
normalized to the vector control reaction. Western blots
detecting T7- and HA-tagged proteins are shown. (A) Activation
of ERK2 by SOS^cat and SOS^cat mutants. Results shown in the bar
diagram are from three independent experiments. Error bars
indicate standard deviations of three independent experiments.
The amout of ^32P incorporation into MBP was quantified by
phosphorimaging. Autoradiograms and Western blots shown are from
a single representative experiment. (B) Activation of ERK2 by
SOS truncations. Results shown are from a single representative
experiment. Experiments were repeated three times with similar
results.
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