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Figure 6.
Fig. 6. Model of 2G12 glycan recognition of gp120. On the basis
of our model, three separate Man[9]GlcNAc[2] moieties, shown in
red (two in the primary combining sites and one in the
V[H]/V[H]' interface), potentially mediate the binding of 2G12
to gp120. The glycans at the primary combining sites originate
from Asn332 and Asn392 (labeled) in gp120, whereas the
carbohydrate located at the V[H]/V[H]' interface would arise
from Asn339 (labeled). N-linked glycans occurring at Asn332 and
Asn392 have previously been implicated as critical for 2G12
binding (13). The N-linked glycan at Asn 339 is not as critical
for 2G12 binding, although this glycan could potentially
interact with the V[H]/V[H]' interface. Figure was generated
using programs Molscript (65) and Raster3D (66).
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