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Figure 6.
Figure 6. Analytical ultracentrifugation data on the first
three Ig domains from N-CAM, collected on a Beckman XL-I
centrifuge. (a) Sedimentation data recorded at 14,000 rpm on an
11 µM solution of wild-type Ig I-III in PBS (o) fitted to
an associating model of monomer-dimer equilibrium (continuous
line). (b) Sedimentation data recorded at 20,000 rpm on a 12
µM solution of F19S Ig I-III in PBS (o) fitted to a single
species (continuous line). The residual differences between the
experimental data and the fit for each point are shown above for
each experiment.
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