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Figure 6.
Figure 6. (a) Superposition of the mutant monomer,
Cro-[DGEVK]-F58W (open bonds), on one subunit of the native Cro
dimer (filled bonds). (b) Superposition of one subunit of the
mutant monomer, Cro-[DGEVK]-F58W (open bonds), on one subunit of
the WT Cro dimer (filled bonds) in its conformation when bound
to operator DNA. (c) Detailed view of (b) in the vicinity of
residue 58 showing the superposition of the native Cro-operator
complex (filled bonds) on one subunit of Cro-F58W. The indole
ring of Trp58 displaces Arg38' from its position in the Phe58
structure and would tend to disrupt the interaction between
Arg38' and the phosphate oxygen atom labeled S12 O2P. This
labeling is as used by the Protein Data Bank. In terms of common
conventions for the Cro system (e.g. see [Albright and Matthews
1998]), S11 O2P and S12 O2P correspond, respectively, to
phosphate groups P[E] and P[D].
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