|
Figure 6.
Figure 6. Model of the α-Actinin Rod(A) Sequence alignment
of the α-actinin repeats used in modeling of repeats R1 and R4.
Residue numbers for the full-length molecule and those of the
construct used for the crystal structure are indicated at the
edges and above the alignment, respectively. The α helices
seen in the crystal structure are depicted as bars (blue for R2
and green for R3). The C termini of R1 and R3 and the N termini
of R2 and R4, respectively, overlap due to the modeling
procedure of R1 and R4. The overlapping residues shown in italic
were used to assemble the model of the rod. The figure was
generated with ALSCRIPT ([1]).(B) Ribbon diagram of α-actinin
rod viewed in two orientations related by a 65 degree rotation
around the long molecular axis through the central 2-fold axis.
R1 is colored violet, R2 is blue, R3 is green, and R4 is
yellow.(C) Electrostatic surface potential of one R1–R4
subunit generated with GRASP ([38]). Positively charged surface
is colored blue and negatively charged red. The second subunit
is white in a wormlike representation. Surfaces corresponding to
R1 and R4 are marked.
|
