|
Figure 5.
Structural analysis of the S31N drug resistant mutant. (A)
Ribbon representation of a structural model of the S31N mutant,
illustrating the 2 lysines used in cross-linking. (B) Results of
structural refinement of the helix–helix interface of
S31N(18–60) based on experimental NOE data. The dashed lines
represent intersubunit NOEs involving the side-chain NH[2] of
Asn-31. For comparison, a similar view of WT(18–60) (PDB,
2RLF) is shown. The side chains of both Ser-31 in the WT and
Asn-31 in the mutant are positioned at the helix–helix
interface. (C) SDS/PAGE of M2(18–60) variants after being
treated with 75 μM DSP for 15 min (for details, see Methods).
(D) Same as in C except 2.5 mM DSP and 60 min of reaction time
were applied.
|
