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Figure 5.
Figure 5. The Interface Between the RGS-Box and C-Terminal
Extension of PRG-rgRGS and Gα13
(A) The solvent accessible
surface of Gα13 is colored as in Figure 1C, with residues
contacting the rgRGS colored blue. The RGS-box and C-terminal
extension is colored green, with elements contacting Gα13
colored red.
(B) Residues of Gα13 that contact PRG-rgRGS
are colored according to electrostatic potential as in Figure
2A. Side chains from PRG-rgRGS that directly contact Gα13 are
represented as ball-and-stick models. In addition to the αE
helix, the α3-α4 and α10-α11 loops of PRG-rgRGS also
directly contact the effector-binding site of Gα13.
(C)
Differences at the effector-binding site on Gα13 upon binding
to PRG-rgRGS (left) or p115-rgRGS (right). Residues directly
involved in the rgRGS:Gα13 interface are represented as
ball-and-stick models.
(D) Ribbon diagram depicting the
interaction interface between switch II of Gα13 and the
N-terminal and the RGS-box subdomains of PRG-rgRGS. Main chain
and side chain atoms are represented as ball-and-stick models.
Hydrogen bonds are drawn as dotted lines.
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