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Figure 5.
Figure 5. PRE Experiment with Spin-Labeled LD1
(A)
Details of the ^1H-^15N HSQC spectra of 230 μM ^15N-enriched
α-parvin-CH[C] and 250 μM PROXYL-labeled LD1 peptide in the
absence (left) and presence (right) of 5 mM ascorbate. The
latter serves to reduce the spin label, thereby eliminating PRE
effects. The binding orientation of LD1 seen in the crystal
structure is denoted “forward.”
(B) Ribbon
representation of α-parvin-CH[C] in gold and LD1 in green. The
position of α-parvin residues 257 and 370 are highlighted in
blue and red, respectively.
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