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Figure 5.
Crystal structure and molecular docking analysis of
TIR1–probe complexes. (A and B) Crystal structure of
TIR1–probe complexes. TIR1 is shown as silver ribbon. Probes
3, 4, and 8 are shown as blue, yellow, and green, respectively.
IAA7 degron peptide (pink, surface-filled model) and IAA (red)
were superimposed on the coordinates in the crystal structure of
the TIR1-IAA-IAA7 complex. (C) Molecular docking of TIR1 probe.
Predicted binding conformers of 3 (blue) and 4 (yellow) to TIR1
auxin-binding site. Fifty possible binding conformers were
predicted by the program AutoDock. Ten representative conformers
were shown based on rmsd values to the coordinates of IAA moiety
in 3 and 4 in crystal structure.
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