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Figure 5.
Figure 5. Sedimentation velocity g(s*) analyses of SCR-1/5
and SCR-16/20 using DCDT+. (a) The 20 absorbance scans for
SCR-1/5 at 0.09 mg/ml and 0.85 mg/ml and a rotor speed of 35,000
r.p.m. gave s^o[20,w] values of 2.50 S and 2.51 S, respectively.
The intensity of the g(s*) curve at 0.09 mg/ml was increased
threefold for clarity. (b) The corresponding 14 and 22
interference scans from the same SCR-1/5 experiment gave
s^o[20,w] values of 2.39 S and 2.49 S, respectively. (c)
Absorbance data for SCR-16/20 at 0.10 mg/ml (40,000 r.p.m.) and
0.62 mg/ml, 1.33 mg/ml and 1.48 mg/ml (50,000 r.p.m.) showed
that the s^o[20,w] values increased from 2.8 S to 3.5 S as the
concentration increased. The intensities of the g(s*) curves
were equalised for reason of clarity. (d) The corresponding
interference data sets for SCR-16/20 showed that the s^o[20,w]
values likewise increased from 2.7 S to 3.5 S as the
concentration increased.
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