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Figure 5.
FIGURE 5. Examples of the quality of the final electron
density. Electron density around the DNA duplex at the active
site is shown. A, Dpo4(DFT:13A); template
5'-TTCAG(DFT)AGTCCTTCCCCC-3' and primer 5'-GGGGGAAGGACTA-3',
ddCTP. B, Dpo4(dGTP); template 5'-TTCA(DFT)TAGTCCTTCCCCC-3' and
primer 5'-GGGGGAAGGACTA-3', dGTP. C, one of the Dpo4(DFT:13G)
complexes per asymmetric unit; D, second Dpo4(DFT:13G) complex;
template 5'-TTCAG(DFT)AGTCCTTCCCCC-3' and primer
5'-GGGGGAAGGACTG-3', ddCTP. The 3F[o] - 2F[c]electron density
maps (blue chicken wire) are contoured at the 1 level.
The Dpo4 protein is shown as ribbons and the DNA duplex as
sticks. The Ca^2+ ions are depicted as green spheres.
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