Figure 5 - full size

 

Figure 5.
FIGURE 5. Examples of the quality of the final electron density. Electron density around the DNA duplex at the active site is shown. A, Dpo4(DFT:13A); template 5'-TTCAG(DFT)AGTCCTTCCCCC-3' and primer 5'-GGGGGAAGGACTA-3', ddCTP. B, Dpo4(dGTP); template 5'-TTCA(DFT)TAGTCCTTCCCCC-3' and primer 5'-GGGGGAAGGACTA-3', dGTP. C, one of the Dpo4(DFT:13G) complexes per asymmetric unit; D, second Dpo4(DFT:13G) complex; template 5'-TTCAG(DFT)AGTCCTTCCCCC-3' and primer 5'-GGGGGAAGGACTG-3', ddCTP. The 3F[o] - 2F[c]electron density maps (blue chicken wire) are contoured at the 1 level. The Dpo4 protein is shown as ribbons and the DNA duplex as sticks. The Ca^2+ ions are depicted as green spheres.

The above figure is reprinted by permission from the ASBMB: J Biol Chem (2007, 282, 36421-36433) copyright 2007.