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Figure 5.
FIGURE 5. Structure of ZO2-PDZ2. A, backbone overlay of 20
NMR structures with the lowest energy from the final CNS v1.1
calculation. This figure was produced with MOLMOL. B, a ribbon
representation of the energy-minimized average structure with
the secondary structure elements labeled. This figure was
produced with PyMOL (W. L. DeLano (2002) PyMOL, DeLano
Scientific, San Carlos, CA); for clarity, the two chains A and B
are colored in green and cyan, with their N and C termini
labeled with N[A], C[A], N[B], and C[B], respectively. C,
comparison of the three-dimensional structure of the GRIP2PDZ3
monomer (PDB entry: 1V62) to that of one subunit in the ZO2-PDZ2
homodimer. The backbones of some secondary structured regions of
the GRIP2PDZ3 monomer (yellow,  1, 2, β3, β4, β5, and
β6) and a subunit of the ZO2-PDZ2 homodimer (green, 1, 2, β2b,
β3, β4, and β5) are superimposed to each other. This figure
was produced with PyMOL. D, sequence alignment of the second PDZ
domains of ZO proteins, GRIP2PDZ3, GRIP1PDZ6, and Shank1PDZ. The
sequences were aligned using the program ESPript (Easy
Sequencing in PostScript) (42). Residues identical in all
sequences are colored white in the red column; conserved
residues are colored red, whereas the others are in black.
Secondary structure elements for ZO2-PDZ2 and Shank1PDZ are
shown above or below the sequences, respectively, with helices
as squiggles, strands as arrows, and turns as TT.
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